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| How to use PSPHunter? |
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To predict phase separation proteins, you need to conduct a four-step procedure as shown in the following figure: (1) Choose the type of submission; (2) Paste or upload your query protein file; (3) Input your email address for retrieving the results (Optional); (4) Click the submit button.
Once your job is successfully submitted, PSPHunter will assign a Job ID to your query. You can check the running status and retrieve your results using this Job ID. Generally, the prediction process will be finished in 15 minutes for a single query. After the job is done, the URL of the results webpage will be returned to you by email. |
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| Format of input file |
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PSPHunter can be used to predict both phase-separating proteins and driving residues from protein sequence. Please input the standard FASTA format as shown in the following figure.
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| Online prediction results |
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Once the prediction process is finished, PSPHunter will provide a webpage to illustrate the prediction results. As shown in the following figure, the results will be displayed from four perspectives. The first section provides summary information about the query protein and its optimal template. Especially, the predicted driving residues are highlighted in red color in the sequence. The second section shows graphical representation of prediction results. The last section includes the details about the prediction results, including the outputs from our proposed different predictors as suggested in the manuscript. You can also download the results from the hyperlink provided in the first section.
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